· polyackolladeGel dole ne ta hanyar monryamid monomer, polymerization polymerization abu, mai kara kuzari, da kuma hakkin gishiri da cakuda cakuda tare.
· na lissafida bis (n, n 'kerrylama biyu ne mai monomer form gel matrix.
· Ammonium nace farawa tsarin polymerization. Tsarin glue yana buƙatar 12% ammonium ferulfate an shirya shi cikin ruwa. Yawancin bayanan da aka nuna buƙatar amfani mai aiki. Koyaya, za'a iya sanya bayani na 10% a cikin 4 ℃ don makonni da yawa ba tare da babban rabo na aiki ba. Yi har zuwa 10 ml da kuma zubar da lokacin da manne ya kasa tara.
Tip: Kashi nana lissafiA glogin gluincing da manne baki ba iri ɗaya bane. Idan amfani da polrylama prerylamide: bis bayani, tabbatar da samun kwalban da ya dace.
Heed (n, N, N '- Tetramethyl Ethynentiamine) shine mai kara kuzari, a cikin kwalban launin ruwan kasa, sanya shi a cikin firiji. Ƙara kawai kafin zubar manne.
Ya kamata a wanke gilashin da aka yi amfani da gilashin gilashi a cikin electrophoresis kafin kuma bayan kowane lokaci. Bayan Olllectrophoreseses, wanke tare da buroshi mai laushi da zane a cikin soapy mai zafi, kurkura tare da distilled ruwa da kuma tsaya bushe.
Juyin danshi da ƙura na iya haifar da m polymer. Kafin Olllerophoresesis, tsaftace farantin gilashin tare da tsabtace gilashi kuma ya goge shi da goga mai taushi. A wanke tare da distilled ruwa da bushe sosai tare da takarda goge. Rinsing tare da 70% ethanol kafin shafa tare da takarda yana taimakawa tsaftace da sauri bushewa. Sanya samfurori na Acrylamide na Acrylami da aka Nasara: BIS, ruwa, maganin buffer, maganin ammonium, nunawa, hemed. Shake da kyau kuma zuba nan da nan.
Ba lallai ba ne a cikin Polyacryledid kafin polymerization. (Acrylaid amfani da shi a cikin wani wuri don cire kumfa saboda oxygen yana hana polymerization polymerization.)
A kwance manne mai ma'ana samfurin.
Rubutun takarda mai baƙar fata a cikin akwati, baƙar fata na asali don ganin rami samfurin don ganin ƙarin rami a sarari.
Dandalin tanki mai haske wanda aka cika shi, kawai akan Colloid.
Idan gefen yana da haske, kunna fitilun, bari haske yana haskakawa Colloid. Zana samfurin a cikin bututun.
Yin amfani da na'urar atistic atomatik.
Ana iya amfani da Points 10-200 1 a yawancin wuraren akan samfurin. Don karamin samfurin samfurin ramuka (ƙasa da 10μ1), dogon buttette kai da aka yi amfani da shi don glue mai kyau shine mafi dacewa.
Kawai nutsuwa kawai yana nutsar da shi a cikin samfurin, shayar da ruwa a hankali. Samfurin na iya bayyana mai wuya tare da glycerin, kuma mai saurin yin famfo na iya jawo kumfa a cikin bututun bututun.
Samesan samfurai bayan shuki butetting kai, za su motsa kai mai ruwa a hankali a gefen bututu ko takarda shafewa yana tsotse ruwa kai a waje da droplets. Yi hankali kada ku tsotse samfurin.
Sanya samfurin a cikin ramin samfurin
Na'urar bututun don ci gaba da matsin lamba, sanya samfurori dan kadan motsin da ke motsa ruwa a kai.
Head bututun bututun a cikin buffer, dan kadan sama da ramuka na tabo, kula da matsi mai kyau. Za'a iya shigar da tip na pipette a cikin karamin rami.
A hankali da kuma daidaita samfurori. Ana sanya tip ɗin bututun mai sama da rami na samfurin, kuma samfurin zai nutse cikin rami. Bari samfurin nutsewa don cika rami samfurin maimakon turawa.
Umplearshen digo na ƙarshe tare da kai tsaye, ruwa zai matsa zuwa kafa na biyu, sannu a hankali ɗaga motsi, a hankali yana motsa ruwa, ya fita daga buffer
Ta yaya za a yi tsaye a tsaye manue?
Ramuka na manne-tsaye na manne-tsaye wanda aka kafa tsakanin gilashin biyu. A cikin farin ciki mai bakin ciki, ba ma iya shigar da bututun bututun mai tsakanin faranti biyu. Kalli glycerin! Sanya bututun bututun a kan ramin samfurin kuma samfurin zai nutse cikin rami.
Alamu na gaba, tabbatar da sanya aya ta tsaye ta Polyl Gel Samfurin kwarara. A wanke da orrylamized acrylamide da ruwa wanda zai iya bayyana a kasan samfurin rami. Ruwa na iya sanya bashin samfurin yana da matukar muhimmanci. Yi amfani da sirinji 25ml ko 50ml da kuma allura 18-18-. Zuba a cikin ƙirar lantarki kuma a hankali a hankali a hankali.
Zai iya zama da wahala a ga ramin samfurin, amma a daidai yake, sauran yana da sauƙi. Idan akwai wuce haddi ramuka, ana iya gwada samfurin samfurin tare da Bromophenol Blue.
Lokacin Post: Mar-31-2023